Metadata

Micro-CT was performed to a Phascolosoma (Phascolosoma) stephensoni specimen. This sample was fixed in formalin and preserved in ethanol. Specimen was stained with 1% Iodine dissolved in 96% ethanol. Scan was performed with a Skyscan 1172 at a voltage of 50kV and 198μΑ without filter for a half rotation of 180^o . This specimen was scanned at the highest camera resolution with an exposure time of 946ms. This scan was performed for the visualisation of the retractor muscles of this specimen within the framework of MAPWORMS project funded from the European Union’s Horizon Europe research and innovation programme under grant agreement N° 101046846 . Specimen was provided by Luigi Musco (CONISMA) and scanned by Niki Keklikoglou.

Micro-CT was performed to a Seriola sp. juvenile. This sample was fixed in formalin and preserved in ethanol. Specimen was stained with 0.3% PTA dissolved in 70% ethanol. Scan was performed with a Skyscan 1172 at a voltage of 49kV and 198μΑ without filter for a full rotation of 360^o . This specimen was scanned at the highest camera resolution with an exposure time of 625ms. This scan was performed for the visualisation of the gastrointestinal system of this specimen. Specimen was provided by Ioannis Papadakis (HCMR) and scanned by Niki Keklikoglou.

Micro-CT was performed to a thrombus specimen. This sample was fixed in formalin and preserved in ethanol. Specimen was stained with 0.3% PTA dissolved in 70% ethanol. Scan was performed with a Skyscan 1172 at a voltage of 50kV and 198μΑ without filter for a full rotation of 360^o . This specimen was scanned at the highest camera resolution with an exposure time of 325ms. This scan was performed for the evaluation of the impact of aspirated thrombus burden on the post‐aspiration clinical and angiographical outcomes of patients with ST‐Elevated Myocardial Infarction (STEMI). Specimen was provided by Efstratios Karagiannidis (AUTH) and scanned by Eva Chatzinikolaou.

Micro-CT was performed to a Drosophila melanogaster. This specimen was fixed in formalin and preserved in 96% ethanol. Specimen was stained with 1% iodine dissolved in 96% ethanol. Scan was performed with a Skyscan 1172 at a voltage of 59kV and 167μΑ without filter for a half rotation of 360^o. This specimen was scanned at the highest camera resolution with an exposure time of 650ms. This scan was performed for visualising the heart of the specimen. Specimen was provided by Ioannis Trougakos and scanned by Niki Keklikoglou.

Micro-CT was performed to a 3D printed scaffold with mesenchymal stem cells. This sample was fixed in formalin and preserved in ethanol. Specimen was stained with 0.3% PTA dissolved in 70% ethanol. Scan was performed with a Skyscan 1172 at a voltage of 50kV and 201μΑ with an aluminium filter for a half rotation of 180^o . This specimen was scanned at the highest camera resolution with an exposure time of 1620ms. This scan was performed for visualising the mesenchymal stem cells on the scaffold. Specimen was provided by Konstantinos Theodoridis (AUTH) and scanned by Niki Keklikoglou.

Micro-CT was performed to an amphipod. This specimen was fixed in formalin and preserved in 96% ethanol. Specimen was stained with 1% iodine dissolvded in 96% ethanol. Scan was performed with a Skyscan 1172 at a voltage of 59kV and 167μΑ without filter for a half rotation of 180^o . This specimen was scanned at the highest camera resolution with an exposure time of 316ms. This scan was performed for visualising the interior characteristics of the specimen. Specimen was provided and scanned by Niki Keklikoglou.

Micro-CT was performed to a polychaete Chrysopetalum debile. This specimen was fixed and preserved in 96% ethanol. For this specimen, no contrast agent was used. Scan was performed with a Skyscan 1172 at a voltage of 60kV and 172μΑ without filter for a full rotation of 360^o . This specimen was scanned at the highest camera resolution with an exposure time of 1915ms. This scan was performed for visualising the jaws and pharynx for comparative morphology. Specimen was provided by Giorgos Chatzigeorgiou and scanned by Sarah Faulwetter.

This scan was performed for the assesment of the effects of climate change and ocean acidification on marine gastropods within the ECCO project (https://ecco.hcmr.gr/). Micro-CT was performed to Hexaplex trunculus gastropods which were maintained under low pH and increased temperature. For this specimen, no contrast agent was used. Scan was performed with a Skyscan 1172 at a voltage of 100kV and 100μΑ with an aluminium and copper filter for a half rotation of 180^o . Images were acquired at a pixel size of 13.79μm with an exposure time of 2480ms. Specimen was provided and scanned by Eva Chatzinikolaou.

Datasets, images and videos are licensed under a Creative Commons Attribution 4.0 International License

How to cite this work: Chatzinikolaou E. (2021) Effect of climate change and ocean acidification on marine gastropods - ECCO project.

This scan was performed for the assesment of the effects of climate change and ocean acidification on marine gastropods within the ECCO project (https://ecco.hcmr.gr/). Micro-CT was performed to Hexaplex trunculus gastropods which were maintained under normal pH and increased temperature. For this specimen, no contrast agent was used. Scan was performed with a Skyscan 1172 at a voltage of 100kV and 100μΑ with an aluminium and copper filter for a half rotation of 180^o . Images were acquired at a pixel size of 13.79μm with an exposure time of 2480ms. Specimen was provided and scanned by Eva Chatzinikolaou.

Datasets, images and videos are licensed under a Creative Commons Attribution 4.0 International License

How to cite this work: Chatzinikolaou E. (2021) Effect of climate change and ocean acidification on marine gastropods - ECCO project.

This scan was performed for the assesment of the effects of climate change and ocean acidification on marine gastropods within the ECCO project (https://ecco.hcmr.gr/). Micro-CT was performed to Hexaplex trunculus gastropods which were maintained under normal pH and ambient temperature. For this specimen, no contrast agent was used. Scan was performed with a Skyscan 1172 at a voltage of 100kV and 100μΑ with an aluminium and copper filter for a half rotation of 180^o . Images were acquired at a pixel size of 13.79μm with an exposure time of 2480ms. Specimen was provided and scanned by Eva Chatzinikolaou.

Datasets, images and videos are licensed under a Creative Commons Attribution 4.0 International License

How to cite this work: Chatzinikolaou E. (2021) Effect of climate change and ocean acidification on marine gastropods - ECCO project.

This scan was performed for the assesment of the effects of climate change and ocean acidification on marine gastropods within the ECCO project (https://ecco.hcmr.gr/). Micro-CT was performed to Hexaplex trunculus gastropods which were maintained under low pH and ambient temperature. For this specimen, no contrast agent was used. Scan was performed with a Skyscan 1172 at a voltage of 100kV and 100μΑ with an aluminium and copper filter for a half rotation of 180^o . Images were acquired at a pixel size of 13.79μm with an exposure time of 2480ms. Specimen was provided and scanned by Eva Chatzinikolaou.

Datasets, images and videos are licensed under a Creative Commons Attribution 4.0 International License

How to cite this work: Chatzinikolaou E. (2021) Effect of climate change and ocean acidification on marine gastropods - ECCO project.

Micro-CT was performed to the skull of a mouse (Mus musculus). This specimen was preserved in 100% ethanol. For this specimen, no contrast agent was used. Scan was performed with a Skyscan 1172 at a voltage of 75kV and 131μΑ with an an aluminium filter of 0.5mm for a half rotation of 180^o . This specimen was scanned at a pixel size of 13.79μm  with an exposure time of 1435ms. This scan was performed to a Erf^loxP/- craniosynostosis animal (http://bioimaginglab.hcmr.gr/#) . Specimen was provided by George Mavrothalassitis (FORTH) and scanned by Niki Keklikoglou.

Micro-CT was performed to the skull of a mouse (Mus musculus). This specimen was preserved in 100% ethanol. For this specimen, no contrast agent was used. Scan was performed with a Skyscan 1172 at a voltage of 75kV and 131μΑ with an an aluminium filter of 0.5mm for a half rotation of 180^o . This specimen was scanned at a pixel size of 13.79μm  with an exposure time of 1435ms. This scan was performed as a control treatment for the craniosynostosis study (http://bioimaginglab.hcmr.gr/#) . Specimen was provided by George Mavrothalassitis (FORTH) and scanned by Niki Keklikoglou.

Micro-CT was performed to a gastropod (Nassarius nitidus ). This specimen was frozen in liquid nitrogen. For this specimen, no contrast agent was used. Scan was performed with a Skyscan 1172 at a voltage of 70kV and 141μΑ with an an aluminium filter of 0.5mm for a half rotation of 180^o . This specimen was scanned at the highest camera resolution with an exposure time of 6455ms. This scan was performed for the assesment of the effects of ocean acidification (http://acidoc.lifewatchgreece.eu/). Specimen was provided by Nafsika Papageorgiou and scanned by Eva Chatzinikolaou.

Micro-CT was performed to a brown algae (Cystoseira sp.). This specimen was fixed and preserved in 96% ethanol. For this specimen, no contrast agent was used. Scan was performed with a Skyscan 1172 at a voltage of 60kV and 167μΑ without filter for a full rotation of 360^o . This specimen was scanned at a medium camera resolution with an exposure time of 460ms. This scan was performed for the visualization of the specimen. Specimen was provided by Giorgos Chatzigeorgiou and scanned by Niki Keklikoglou.

Micro-CT was performed to the anterior end of a polychaete (Syllis gracilis). This specimen was fixed in formalin and preserved in 96% ethanol. Specimen was stained in 1% Iodine solution in 96% ethanol for 3 days. Scan was performed with a Skyscan 1172 at a voltage of 59kV and 167μΑ without filter for a full rotation of 360^o . This specimen was scanned at the highest camera resolution with an exposure time of 1165ms. This scan was performed for the visualization of the pharynx of the specimen (http://zookeys.pensoft.net/articles.php?id=3650). Specimen was provided and scanned by Sarah Faulwetter. The original dataset is available in https://datadryad.org/resource/doi:10.5061/dryad.84m54.

Micro-CT was performed to the anterior end of a polychaete (Phyllodoce sp.). This specimen was fixed in formalin and preserved in 96% ethanol. Specimen was stained in 0.3% PTA solution in 70% ethanol for 36 hours. Scan was performed with a Skyscan 1172 at a voltage of 59kV and 167μΑ without filter for a full rotation of 360^o . This specimen was scanned at the highest camera resolution with an exposure time of 1910ms. This scan was performed for the visualization of the pharynx of the specimen (http://zookeys.pensoft.net/articles.php?id=3650). Specimen was provided by Maria Maintanou and scanned by Sarah Faulwetter. The original dataset is available in https://datadryad.org/resource/doi:10.5061/dryad.84m54.

Micro-CT was performed to the anterior end of a polychaete (Alitta succinea). This specimen was fixed and preserved in 96% ethanol. Specimen was stained in 0.3% PTA solution in 70% ethanol for 20 days. Scan was performed with a Skyscan 1172 at a voltage of 59kV and 167μΑ without filter for a full rotation of 360^o . This specimen was scanned at a medium camera resolution with an exposure time of 190ms. This scan was performed for the visualization of the everted pharynx of the specimen (http://zookeys.pensoft.net/articles.php?id=3650). Specimen was provided by Katerina Vasileiadou and scanned by Sarah Faulwetter. The original dataset is available in https://datadryad.org/resource/doi:10.5061/dryad.84m54.

Micro-CT was performed to the anterior end of a polychaete (Phyllodoce lineata). This specimen was fixed in formalin and preserved in 96% ethanol. Specimen was stained in 0.3% PTA solution in 70% ethanol for 36 hours. Scan was performed with a Skyscan 1172 at a voltage of 59kV and 167μΑ without filter for a full rotation of 360^o . This specimen was scanned at the highest camera resolution with an exposure time of 1165ms. This scan was performed for the visualization of the everted pharynx of the specimen (http://zookeys.pensoft.net/articles.php?id=3650). Specimen was provided by Maria Maintanou and scanned by Sarah Faulwetter. The original dataset is available in https://datadryad.org/resource/doi:10.5061/dryad.84m54.

Micro-CT was performed to the anterior end of a polychaete (Lumbrineris latreilli). This specimen was fixed in formalin and preserved in 96% ethanol. For this specimen, no contrast agent was used. Scan was performed with a Skyscan 1172 at a voltage of 60kV and 172μΑ without filter for a full rotation of 360^o . This specimen was scanned at a medium camera resolution with an exposure time of 1925ms. This scan was performed for the visualization of the specimen (http://zookeys.pensoft.net/articles.php?id=3650). Specimen was provided by Maria Maintanou and scanned by Sarah Faulwetter. The original dataset is available in https://datadryad.org/resource/doi:10.5061/dryad.84m54.

Micro-CT was performed to a polychaete (Hermodice carunculata). This specimen was fixed and preserved in 96% ethanol. Specimen was dried with HMDS. Scan was performed with a Skyscan 1172 at a voltage of 40kV and 250μΑ without filter for a full rotation of 360^o . This specimen was scanned at a medium camera resolution with an exposure time of 155ms. This scan was performed for testing contrast enhancing techniques (http://zookeys.pensoft.net/articles.php?id=3650). Specimen was provided and scanned by Sarah Faulwetter. The original dataset is available in https://datadryad.org/resource/doi:10.5061/dryad.84m54.

Micro-CT was performed to a juvenile polychaete (Eunice sp.). This specimen was fixed and preserved in 96% ethanol. Specimen was stained in 0.3% PTA solution in 70% ethanol for 24 hours. Scan was performed with a Skyscan 1172 at a voltage of 59kV and 167μΑ without filter for a full rotation of 360^o . This specimen was scanned at the highest camera resolution with an exposure time of 1835ms. This scan was performed for testing contrast enhancing techniques (http://zookeys.pensoft.net/articles.php?id=3650). Specimen was provided and scanned by Sarah Faulwetter. The original dataset is available in https://datadryad.org/resource/doi:10.5061/dryad.84m54 .

Micro-CT was performed to a juvenile polychaete (Eunice sp.). This specimen was fixed and preserved in 96% ethanol. Specimen was stained in 1% Iodine solution in 96% ethanol for 24 hours. Scan was performed with a Skyscan 1172 at a voltage of 59kV and 167μΑ without filter for a full rotation of 360^o . This specimen was scanned at the highest camera resolution with an exposure time of 1835ms. This scan was performed for a visualization of chaetae (http://zookeys.pensoft.net/articles.php?id=3650). Specimen was provided and scanned by Sarah Faulwetter. The original dataset is available in https://datadryad.org/resource/doi:10.5061/dryad.84m54.

Micro-CT was performed to a parapod of Eunice roussaei (Polychaeta). This specimen was fixed and preserved in formalin. Specimen was dried with HMDS for 2 hours. Scan was performed with a Skyscan 1172 at a voltage of 60kV and 167μΑ without filter for a full rotation of 360^o . This specimen was scanned at the highest camera resolution with an exposure time of 1155ms. This scan was performed for a visualization of chaetae (http://zookeys.pensoft.net/articles.php?id=3650). Specimen was provided by Aristotle University of Thessaloniki (AUTH) and scanned by Sarah Faulwetter. The original dataset is available in https://datadryad.org/resource/doi:10.5061/dryad.84m54.

Micro-CT was performed to an ophiuroid (Ophiuroidea, Echinodermata). This specimen was fixed and preserved in 96% ethanol. For this specimen, no contrast agent was used. Scan was performed with a Skyscan 1172 at a voltage of 40kV and 250μΑ without filter for a full rotation of 360^o . This specimen was scanned at a medium camera resolution with an exposure time of 165ms. This scan was performed for testing visualization techniques of several marine organisms. Specimen was provided and scanned by Sarah Faulwetter.

Micro-CT was performed to a small sea urchin (Echinoidea, Echinodermata). This specimen was fixed and preserved in 96% ethanol. For this specimen, no contrast agent was used. Scan was performed with a Skyscan 1172 at a voltage of 48kV and 200μΑ with an aluminium filter of 0.5mm for a full rotation of 360^o . This specimen was scanned at a medium camera resolution with an exposure time of 500ms. This scan was performed for testing visualization techniques of several marine organisms. Specimen was provided and scanned by Sarah Faulwetter.

Micro-CT was performed to a copepod which belongs to Calanus genus. This specimen was fixed and preserved in 96% ethanol. Specimen was stained in 0.3% PTA solution in 70% ethanol. Scan was performed with a Skyscan 1172 at a voltage of 59kV and 167μΑ without filter for a full rotation of 360^o . This specimen was scanned at the highest camera resolution with an exposure time of 660ms. This scan was performed for testing visualization techniques of several marine organisms. Specimen was provided by Panagiotis Kasapidis and scanned by Sarah Faulwetter.

Micro-CT was performed to a small fish which belongs to Sparidae family. For this specimen, no contrast agent was used. Scan was performed with a Skyscan 1172 at a voltage of 44kV and 222μΑ with a custom made aluminium filter (two layers of aluminium foil) for a full rotation of 360^o . This specimen was scanned at a medium camera resolution with an exposure time of 340ms. This scan was performed for testing visualization techniques of several marine organisms. Specimen was provided by Nikos Nikolioudakis and scanned by Jeroen Hostens.

Micro-CT was performed to an isopod. This specimen was fixed and preserved in 96% ethanol. Specimen was dried with HMDS. Scan was performed with a Skyscan 1172 at a voltage of 40kV and 250μΑ without filter for a full rotation of 360^o . This specimen was scanned at the highest camera resolution with an exposure time of 455ms. This scan was performed for testing visualization techniques of several marine organisms. Specimen was provided and scanned by Sarah Faulwetter.

Micro-CT was performed to a small bivalve Musculus costulatus. This specimen was fixed and preserved in 96% ethanol. Specimen was stained in 0.3% PTA solution in 70% ethanol for 5 days. Scan was performed with a Skyscan 1172 at a voltage of 59kV and 167μΑ without filter for a full rotation of 360^o . This specimen was scanned at the highest camera resolution with an exposure time of 1555ms. This scan was performed for testing contrast enhancing techniques for the application of micro-CT in marine biodiversity studies (http://www.microscopy-analysis.com/sites/default/files/2013_March_Faulw…). Specimen was provided and scanned by Sarah Faulwetter.